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MiR-21-5p Protects Embryonic Growth and Heart Function During Developmental Hypoxia by Dampening HIF Responses and Altering Gene Expression

Fetal hypoxia is a common complication of pregnancy that increases cardiovascular risk in offspring. This study tests whether miR-21-5p upregulation during developmental hypoxia protects embryonic growth and cardiac function. Using neonatal rat …

A multi-omic, spatial, and whole-slide image dataset of lung neuroendocrine tumours from the lungNENomics cohort

This preprint presents a multi-omic dataset for lung neuroendocrine tumours from the lungNENomics cohort. The resource includes RNA sequencing, EPIC 850K methylation arrays, whole-genome sequencing, multi-regional sequencing, spatial proteomics, …

A recent and rapid genome expansion driven by the amplification of transposable elements in the Neotropical annual killifish Garcialebias charrua

By comparing two annual killifish genomes, this study shows that Garcialebias charrua experienced a recent and unusually large genome expansion driven mainly by repetitive elements. The analysis links transposable element bursts with genome …

Accurate haplotype-resolved de novo assembly of human genomes with RFhap

RFhap is a reproducible workflow for haplotype-resolved de novo assembly of human genomes using parental short reads and child long reads. The method assigns long reads to parental haplotypes and evaluates downstream assemblies with haplotype-aware …

Transcriptional regulatory network analysis uncovers modular gene control and potential key regulators in diabetic cardiomyopathy

This study reconstructs transcriptional regulatory networks in diabetic cardiomyopathy to identify modular gene-control programs and candidate regulatory drivers. The analysis highlights coordinated transcriptional modules linked to disease biology …

Ultra-deep sequencing reveals intra-host diversity and co-infection-driven evolution of SARS-CoV-2

This preprint uses ultra-deep whole-genome sequencing of SARS-CoV-2 samples from Chile to characterize intra-host variation, low-frequency mutations, co-infections, and variant-level evolutionary dynamics. The study shows how high-depth viral …

A clinically relevant morpho-molecular classification of lung neuroendocrine tumours

This preprint integrates genomics, transcriptomics, methylation, spatial profiling, morphology, and clinical data to refine the classification of lung neuroendocrine tumours. The proposed morpho-molecular groups expose clinically relevant tumour …

Effects of Willow and Pine tree growth in bacterial abundance, interactions and metabolic profile in a copper mine tailing

This preprint examines how willow and pine growth alters the physicochemical and microbial environment of copper mine tailing soils. Vegetated sites showed reduced copper stress, more neutral pH, shifts in bacterial abundance and interaction …

Genome-resolved metagenomics and evolutionary analysis reveal conserved metabolic adaptations in extremophile communities from a copper mining tailing

This study applies genome-resolved metagenomics to microbial communities from a copper mining tailing in central Chile. By reconstructing metagenome-assembled genomes and analyzing metabolic pathways and selection signals, the work identifies …

Impact of Amerindian ancestry on clinical outcomes in Crohn's disease and ulcerative colitis in a Latino population

This study analyzes the relationship between Amerindian ancestry and clinical outcomes in a Latino inflammatory bowel disease cohort. The results suggest that ancestry proportions are associated with distinct Crohn's disease and ulcerative colitis …