Sequencing facility
Sequencing genomes and epigenomes at UOH
Di Genoma Lab operates a long-read sequencing facility centered on Oxford Nanopore technology. Our current platforms include GridION and P2 Solo, which allow us to generate long reads for whole-genome sequencing, targeted sequencing, direct RNA applications, and epigenomic profiling.
Long-read sequencing is critical for the questions we work on. It enables us to detect structural variation, resolve repetitive genomic regions, phase haplotypes, reconstruct high-quality assemblies, and investigate regulatory or epigenetic changes that are difficult to study with short-read data alone.
Research use cases
Our sequencing capacity supports several active research directions:
- cancer genomics and structural variation analysis
- genome assembly and haplotype-aware reconstruction
- Chilean population genomics and genomic diversity studies
- comparative genomics, metagenomics, and evolutionary analyses
- DNA methylation and other long-read-enabled epigenomic applications
The facility is tightly integrated with our local HPC infrastructure, so sequencing projects can move directly into reproducible downstream analysis on Kütral without depending on external compute resources.
| Machine | GridION | P2 Solo |
|---|---|---|
| Sequencing technology | Nanopore | Nanopore |
| Max read length | 2 Mb | 2 Mb |
| Output per run | 10 to 100 Gb | 50 to 580 Gb |
| Sequencing time | 48 to 72 hours | 10 to 24 hours |
| Accuracy | >99% | >99% |
| Applications | Long-read sequencing, de novo assembly, structural variation detection, direct RNA sequencing | Long-read sequencing, de novo assembly, targeted sequencing |
